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Year : 2014  |  Volume : 10  |  Issue : 39  |  Page : 318-324

Methyl protodioscin induces G2/M cell cycle arrest and apoptosis in A549 human lung cancer cells

1 Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
2 Institute of Biology, Guizhou Academy of Sciences, Guiyang, China
3 First Affiliated Hospital of Sun Yat Sen University, Guangzhou, China

Correspondence Address:
Qiang Jia
Institute of Biology, Guizhou Academy of Sciences, Guiyang - 550 009
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0973-1296.137373

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Background: Methyl protodioscin (MPD) is a furostanol bisglycoside with antitumor properties. It has been shown to reduce proliferation, cause cell cycle arrest. Objective: The present study elucidates the mechanism underlying MPD's apoptotic effects, using the A549 human lung cancer cell line. Materials and Methods: The human pulmonary adenocarcinoma cell line A549 was obtained from the Cell Bank of the Animal Experiment Center, North School Region, Sun Yat-Sen University. All of the cells were grown in RPMI 1640 supplemented with 10% fetal calf serum (Hyclone, Logan, UT, USA), penicillin (10,000 U/l), and streptomycin (100 mg/l) at 37°C in a 5% CO 2 humidified atmosphere. The induction of apoptosis was observed in flow cytometry and fluorescent staining experiments. Results: MPD showed growth inhibitory effects in A549 cells in a dose- and time-dependent manner. The significant G2/M cell cycle arrest and apoptotic effect were also seen in A549 cells treated with MPD. MPD-induced apoptosis was accompanied by a significant reduction of mitochondrial membrane potential, release of mitochondrial cytochrome c to cytosol, activation of caspase-3, downregulation of Bcl-2, p-Bad, and upregulation of Bax. Conclusion: Our results show that the induction of apoptosis by MPD involves multiple molecular pathways and strongly suggest that Bcl-2 family proteins signaling pathways. In addition, mitochondrial membrane potential, mitochondrial cytochrome c and caspase-3 were also closely associated with MPD-induced apoptotic process in human A549 cells.

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