|Year : 2014 | Volume
| Issue : 38 | Page : 430-433
Cytotoxicity of gypsogenic acid isolated from Gypsophila trichotoma
Ilina Krasteva1, Maya Yotova1, Deyan Yosifov2, Niko Benbassat1, Kristina Jenett-Siems3, Spiro Konstantinov2
1 Department of Pharmacognosy, Faculty of Pharmacy, Medical University, Sofia, Bulgaria
2 Laboratory for Experimental Chemotherapy, Department of Pharmacology, Pharmacotherapy and Toxicology, Faculty of Pharmacy, Medical University, Sofia, Bulgaria
3 2Institute of Pharmacy, Free University Berlin, Berlin, Germany
|Date of Submission||18-Dec-2013|
|Date of Acceptance||17-Jan-2013|
|Date of Web Publication||28-May-2014|
Department of Pharmacognosy, Faculty of Pharmacy, 2 Dunav St., 1000, Sofia, Bulgaria
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Background: Gypsophila trichotoma Wend. (Caryophyllaceae) is a medicinal plant which is protected in Bulgaria by the Biodiversity Law. Previous studies have showed the presence of triterpene saponins, sterols, flavonoids, triterpens, etc. Objective: Gypsogenic acid, isolated from Gypsophila trichotoma roots, was evaluated for cytotoxic activity. Materials and Methods: The structure of the compound was elucidated by spectral methods. The cell survival fraction was determined by the MTT dye reduction assay, performed with some modifications. Results: Gypsogenic acid was tested in a panel of human tumor cell lines and was found to inhibit the proliferation of malignant cells. It was active against leukemic cells with lymphoid (SKW-3 and BV-173) or myeloid phenotype (HL-60, K-562, and LAMA-84), as well as against the EJ bladder carcinoma cell line. Bcr-Abl expressing myeloid cells (LAMA-84 and especially K-562) displayed lower sensitivity. HL-60/Dox cells were less sensitive to gypsogenic acid than the parent cell line, which shows that gypsogenic acid is probably a substrate of MRP-1.
Keywords: Cytotoxic activity, gypsogenic acid, Gypsophila trichotoma
|How to cite this article:|
Krasteva I, Yotova M, Yosifov D, Benbassat N, Jenett-Siems K, Konstantinov S. Cytotoxicity of gypsogenic acid isolated from Gypsophila trichotoma. Phcog Mag 2014;10, Suppl S2:430-3
|How to cite this URL:|
Krasteva I, Yotova M, Yosifov D, Benbassat N, Jenett-Siems K, Konstantinov S. Cytotoxicity of gypsogenic acid isolated from Gypsophila trichotoma. Phcog Mag [serial online] 2014 [cited 2022 Dec 8];10, Suppl S2:430-3. Available from: http://www.phcog.com/text.asp?2014/10/38/430/133299
| Introduction|| |
Triterpenes comprise one of the most interesting groups of natural products due to their high potential as pharmacological agents. They have been described as anti-inflammatory, antiangiogenic, antiviral, antioxidant, antibacterial, anticancer agents, as well as being immunomodulator compounds.  In the literature available, there are insufficient data about the pharmacological properties of gypsogenic acid. Lee et al.,  isolated this compound from Aceriphyllum rossii and evaluated it for in vitro cytotoxicity against the K-562 and HL-60 cell lines. 
Gypsophila species have been found to accumulate many saponins including both monodesmosidic and bidesmosidic triterpene saponins of gypsogenin, oleanolic acid, quillaic acid, etc.  Some of them have and gypsogenic acid as sapogenin. ,,,,, Triterpenes can be found rarely in the genus and they occur usually as saponins. Only a few have been isolated in native form from the plants of Gypsophila. , The presence of a gypsogenic acid has been reported for G. oldhamiana.  In our previous investigation we isolated from G. trichotoma three triterpenes, including gypsogenic acid. 
Gypsophila trichotoma is a perennial herbaceous plant located in Southeastern Europe, Southwestern Asia, Kazakhstan, and Turkmenistan. The plant is found along the Black Sea coast in Bulgaria. Our previous studies of G. trichotoma resulted in the isolation of saponins, flavonoids, sterols, triterpenes, etc. ,, The hepatoprotective activity of the flavon glycoside saponarin was studied too.  In continuation of our investigations of G. trichotoma, in this paper we describe the cytotoxic activity of gypsogenic acid, isolated from the roots of the species, against six human tumor cell lines.
| Materials and methods|| |
General experimental procedure
1 H Nuclear Magnetic Resonance (NMR) (400 MHz) and 13 C NMR (100.6 MHz) spectra were recorded on Bruker DPX-400 using transcranial magnetic stimulation (TMS) as internal standard. All spectra were recorded in CD 3 OD. High-resolution electron spray ionization mass spectra (HR-ESIMS) was carried out on Agilent 6210 ESI-TOF (Agilent Technology) mass spectrometer. Thin layer chromatography (TLC) study was carried out on silica gel plates (Kieselgel 60 F 254 , Merck) using solvent systems n-BuOH/AcOH/H 2 O (4:1:1) and CHCl 3 /MeOH (9:1). The spots were visualized by spraying anysaldehyde/conc. H 2 SO 4 reagent, followed by heating at 110°C. Chromatography (CC) was carried out with Diaion HP-20 (Supelco) and silica gel 60 (40-60 μm, Merck).
The roots of G. trichotoma were collected in August 2008 at the Black Sea coast, Bulgaria. A voucher specimen (SO 103887) was deposited at the Herbarium of the Faculty of Biology, Sofia University.
Extraction and isolation
Air-dried powdered plant material of G. trichotoma (740 g) was exhaustively extracted with 80% methanol. After partial evaporation the aqueous solutions were extracted with CH 2 Cl 2 , EtOAc, and n-BuOH successively. The residue from the n-BuOH layer was separated by CC on a Diaion HP-20 column, using H 2 O/MeOH (100:0 → 0:100) and further purified by flash chromatography over silica gel with CH 2 Cl 2 /MeOH/H 2 O (18:11:1) to yield gypsogenic acid (50 mg) [Figure 1]. The structure of the compound was determined based on the spectral evidence (HRESI-MS, 1 H and 13 C NMR, correlation spectroscopy (COSY), heteronuclear single-quantum correlation (HSQC), heteronuclear multiple bond correlation (HMBC) experiments). 
Cell lines and culture conditions
The following human cell lines were used: EJ (urinary bladder carcinoma), SKW-3 (T-cell leukemia), BV-173, K-562 and LAMA-84 (chronic myeloid leukemia), and HL-60 (acute myeloid leukemia) and its resistant variant HL-60/Dox which is characterized by the expression of the multi-drug resistance-associated protein MRP-1. All leukemic cell lines were obtained from DSMZ (Braunschweig, Germany) and EJ cells were obtained from the American Type Culture Collection (Rockville, MD, USA). All cells were grown in RPMI-1640 medium supplemented with 10% fetal calf serum (FCS) and 2 mM L-glutamine (all from Lonza, Belgium) under standard conditions (37°C in an incubator with humidified atmosphere containing 5% CO 2 ). The cell cultures were supplemented with fresh medium two or three times per week to maintain them in log phase. HL-60/Dox cells were maintained in medium containing 0.2 μM doxorubicin in order to sustain their multidrug-resistance (MDR) phenotype. One week prior to cytotoxicity determination however, they were kept in drug-free medium in order to avoid synergistic interaction between doxorubicin and the tested compound.
MTT assay for cell survival and proliferation
Exponentially growing cells were seeded into 96-well microplates (100 μl/well at a density of 2 × 10 5 cells/ml for leukemic cells or 5 × 10 4 for EJ cells) and exposed to various concentrations of gypsogenic acid for 72 h. The cell survival fraction was determined by the MTT (3- [4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazoliumbromide) dye reduction assay,  performed with some modifications.  Briefly, after incubation with the test compound, MTT solution (10 mg/ml in PBS) was added (10 μl/well). Plates were further incubated for 3 h at 37ºC and the formazan crystals formed were dissolved by addition of 110 μl solvent (5% formic acid in 2-propanol) per well and mixing. Absorption was measured by an automated microtiter plate spectrophotometer (Labexim LMR-1, Lengau, Austria) at 550 nm. For each concentration at least four wells were used. Complete medium (100 μl), MTT solution (10 μl) and 5% formic acid in 2-propanol (110 μl) were used as blank solution.
Statistics and evaluation of cytotoxic effects
Cell survival fractions were calculated as percentages of respective untreated controls, taken for 100%. Using the GraphPad Prism 5.01 program (GraphPad Software, San Diego, California, USA), concentration-effect curves was fitted, which were then used to interpolate respective IC 50 values and their 95% confidence intervals (CI).
| Results and discussion|| |
Fractionation of methanolic extract, obtained from the roots of G. trichotoma, by a combination of CC over Diaion HP-20 and silica gel resulted in the isolation of gypsogenic acid. 
Gypsogenic acid has previously been shown to possess antibacterial and trypanocidal activity. It inhibited the growth of six cariogenic gram-positive bacterial strains  and was active against blood trypomastigote forms of Trypanosoma cruzi.  Gypsogenic acid was also found to have antihepatotoxic activity in a CCl 4 -based model of liver injury.  In the present study, gypsogenic acid was evaluated for cytotoxic activity against a panel of human tumor cell lines and concentration-dependent cytotoxic effects were observed [Figure 2] and [Figure 3]. BV-173 cells were most sensitive (IC 50 = 41.4 μM; 95% CI: 38.6-44.3 μM), HL-60 cells ranked second (IC 50 = 61.1 μM; 95% CI: 57.7-64.7 μM), and SKW-3 third (IC 50 = 81.5 μM; 95% CI: 79.1-84.0 μM). Gypsogenic acid had similar efficacy against HL-60/Dox, LAMA-84, and EJ cells with IC 50 values ranging between 100 and 125 μM. K-562 cells were the least sensitive to gypsogenic acid (IC 50 = 227.6 μM; 95% CI: 212.6-243.7 μM). Our data for the K-562 cell line are in accordance with those obtained by Lee et al.,  who studied gypsogenic acid isolated from A. rossii, while HL-60 cells were more sensitive in our experiment.
|Figure 2: Survival of (a) BV-173, (b) SKW-3, and (c) EJ, tumor cells after exposure to gypsogenic acid for 72 h. Cell survival fractions were measured using the MTT dye reduction assay and are given as percentages of the respective untreated controls. Bars denote standard deviation|
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|Figure 3: Survival of (a) HL-60, (b) HL-60/Dox, (c) LAMA-84, and (d) K-562, tumor cells after exposure to gypsogenic acid for 72 h. Cell survival fractions were measured using the MTT dye reduction assay and are given as percentages of the respective untreated controls. Bars denote standard deviation|
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| Conclusion|| |
In summary, gypsogenic acid isolated from G. trichotoma exhibited moderate cytotoxic activity against leukemic cells with lymphoid (SKW-3 and BV-173) or myeloid phenotype (HL-60, K-562, and LAMA-84), as well as against the EJ bladder carcinoma cell line. Bcr-Abl expressing myeloid cells (LAMA-84 and especially K-562) displayed lower sensitivity. HL-60/Dox cells were less sensitive to gypsogenic acid than the parent cell line, which shows that the compound is probably a substrate of MRP-1.
| References|| |
|1.||Laszczyk MN. Pentacyclic triterpenes of the lupane, oleanane and ursane group as tools in cancer therapy. Planta Med 2009;75:1549-60. |
|2.||Lee I, Yoo JK, Na M, Min BS, Lee J, Yun BS, et al. Cytotoxicity of triterpenes isolated from Aceriphyllum rossii. Chem Pharm Bull (Tokyo) 2007;55:1376-8. |
|3.||Yotova M, Krasteva I, Nikolov S. Triterpenoid saponins from genus Gypsophila L. (Caryophyllaceae) In: Koh R, Tay I, editors. Saponins: Properties, Applications and Health Benefits. United States: Nova Publishers; 2012. p. 99-122. |
|4.||Böttger S, Melzig MF. Triterpenoid saponins of the Caryophyllaceae and Illecebraceae family. Phytochem Lett 2011;4:59-68. |
|5.||Elbandy M, Miyamoto T, Lacaille-Dubois MA. New triterpenoidal saponins from Gypsophila repens. Helv Chim Acta 2007;90:260-70. |
|6.||Elgamal MH, Soliman HS, Karawya MS, Duddeck H. Isolation of two triterpene saponins from Gypsophila capillaris0 (Forssk.). Nat Prod Lett 1994;4:217-22. |
|7.||Elgamal MH, Soliman HS, Karawya MS, Mikhova B, Duddeck H. Isolation of triterpene saponins from Gypsophila capillaris. Phytochemistry 1995;38:1481-5. |
|8.||Nie W, Luo JG, Kong LY. New triterpenoid saponins from the roots of Gypsophila pacifica Kom. Carbohydr Res 2010;345:68-73. |
|9.||Borges Del Castillo J, Zeitoun B, Arriaga F, Vazquez P. Steryl glucosides of Gypsophila struthium. Fitoterapia 1986;57:61-4. |
|10.||Luo JG, Liu J, Kong LY. New pentacyclic triterpenes from Gypsophila oldhamiana and their biological evaluation as glycogen phosphorylase inhibitors. Chem Biodivers 2008;5:751-7. |
|11.||Yotova M, Krasteva I, Jenett-Siems K, Zdraveva P, Nikolov S. Triterpenoids in Gypsophila trichotoma Wend. Phytochem Lett 2012;5:752-5. |
|12.||Krasteva IN, Popov IS, Balabanova VI, Nikolov SD, Pencheva IP. Phytochemical study of Gypsophila trichotoma Wend. (Caryophyllaceae). Quim Nova 2008;31:1125-6. |
|13.||Krasteva I, Jenett-Siems K, Kaloga M, Nikolov S. 3-O-Sulfo-triterpenoid saponins from Gypsophila trichotoma Wend. Z Naturforsch B 2009;64:319-22. |
|14.||Vitcheva V, Simeonova R, Krasteva I, Yotova M, Nikolov S, Mitcheva M. Hepatoprotective effects of saponarin, isolated from Gypsophila trichotoma Wend. On cocaine-induced oxidative stress in rats. Redox Rep 2011;16:56-60. |
|15.||Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63. |
|16.||Yosifov DY, Todorov PT, Zaharieva MM, Georgiev KD, Pilicheva BA, Konstantinov SM, et al. Erucylphospho-N, N,N-trimethylpropylammonium (erufosine) is a potential antimyeloma drug devoid of myelotoxicity. Cancer Chemother Pharmacol 2011;67:13-25. |
|17.||Scalon Cunha LC, Andrade de Silva ML, Cardoso Furtado NA, Vinhólis AH, Gomes Martins CH, da Silva Filho AA, et al. Antibacterial activity of triterpene acids and semi-synthetic derivatives against oral pathogens. Z Naturforsch C 2007;62:668-72. |
|18.||Cunha WR, Martins C, da Silva Ferreira D, Crotti AE, Lopes NP, Albuquerque S. In vitro trypanocidal activity of triterpenes from miconia species. Planta Med 2003;69:470-2. |
|19.||Hikino H, Ohsawa T, Kiso Y, Oshima Y. Analgesic and antihepatotoxic actions of dianosides, triterpenoid saponins of Dianthus superbus var. longicalycinus herbs. Planta Med 1984;50:353-5. |
[Figure 1], [Figure 2], [Figure 3]
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