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Year : 2013  |  Volume : 9  |  Issue : 35  |  Page : 231-237

Antiproliferative and apoptotic effects of spanish honeys

Department of Food Nutrition and Technology, Veterinarian Faculty, Complutense University, Madrid, Spain

Correspondence Address:
Ana Isabel Haza
Department of Food Nutrition and Technology, Veterinarian Faculty, Complutense University, Madrid
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Source of Support: supported by Grant AGL2008-00292/ALI from the Ministerio de Ciencia e Innovación (Spain) and by Grant 910177 from Programa de Creación y Consolidación de Grupos de Investigación BSCHUCM., Conflict of Interest: None

DOI: 10.4103/0973-1296.113276

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Background: Current evidence supports that consumption of polyphenols has beneficial effects against numerous diseases mostly associated with their antioxidant activity. Honey is a good source of antioxidants since it contains a great variety of phenolic compounds. Objective: The main objective of this work was to investigate the antiproliferative and apoptotic effects of three crude commercial honeys of different floral origin (heather, rosemary and polyfloral honey) from Madrid Autonomic Community (Spain) as well as of an artificial honey in human peripheral blood promyelocytic leukemia cells (HL-60). Material and Methods: HL-60 cells were cultured in the presence of honeys at various concentrations for up to 72 hours and the percentage of cell viability was evaluated by MTT assay. Apoptotic cells were identified by chromatin condensation and flow cytometry analysis. ROS production was determined using 2΄,7΄-dichlorodihydrofluorescein diacetate (H 2 DCFDA). Results: The three types of crude commercial honey induced apoptosis in a concentration and time dependent-manner. In addition, honeys with the higher phenolic content, heather and polyfloral, were the most effective to induce apoptosis in HL-60 cells. However, honeys did not generate reactive oxygen species (ROS) and N-acetyl-L-cysteine (NAC) could not block honeys-induced apoptosis in HL-60 cells. Conclusion: These data support that honeys induced apoptosis in HL-60 cells through a ROS-independent cell death pathway.Moreover, our findings indicate that the antiproliferative and apoptotic effects of honey varied according to the floral origin and the phenolic content.

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