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Simultaneous determination of eleven characteristic lignans in Schisandra chinensis by high-performance liquid chromatography
Junyang Hu1, Chunqin Mao1, Xiaodong Gong1, Tulin Lu2, Han Chen1, Zhijun Huang3, Baochang Cai4
1 College of Pharmaceutical Science, Nanjing University of Chinese Medicine, Nanjing, 210046, China
2 College of Pharmaceutical Science, Nanjing University of Chinese Medicine, Nanjing, 210046; Jiangsu Province Key Research Laboratory of Chinese Medicine Processing, Nanjing, 210046, China
3 Jiangsu Province Key Laboratory of Acupuncture of Nanjing University of Chinese Medicine, Nanjing, 210046, China
4 Jiangsu Province Key Research Laboratory of Chinese Medicine Processing, Nanjing, 210046, China
Correspondence Address:
Tulin Lu
College of Pharmaceutical Science, Nanjing University of Chinese Medicine, Nanjing, 210046
China
 Source of Support: Major Program of State Commission of Science Technology of China O.2009ZX09308-004), Educational Commission of Jiangsu Province of China (NO.09KJA360001), and Key Laboratory of Acupuncture of Jiangsu Province of China (NO.KJA200906)., Conflict of Interest: None  | Check |
DOI: 10.4103/0973-1296.111280
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Background: Schisandra chinensis, one of the well-known traditional Chinese herbal medicines, is derived from the dry ripe fruits of Schisandra chinensis (Turcz.) Baill. according to the 9th China Pharmacopeia. Lignans are the main components isolated from extracts of S. chinensis and their content varies depending on where S. chinensis was collected. We have established a qualitative and quantitative method based on the bioactive lignans for control of the quality of S. chinensis from different sources. Materials and Methods: To develop a high-performance liquid chromatography method, an Elite ODS C18 column (250 mm Χ 4.6 mm, 5μm) at a column temperature of 30°C and flow rate of 1.0ml/min using acetonitrile (A) and water (B) as the mobile phase with a linear gradient and the peaks were monitored at 217 nm. Results: All calibration curves showed good linearity (r ≥ 0.9995) within test ranges. This method showed good repeatability for the quantification of these eleven components in S. chinensis with intra- and inter-day relative standard deviations less than 0.43% and 1.21%, respectively. In the recovery test, results of accuracy ranged from 99.51% to 101.31% with RSD values less than 2.68%, Conclusion: The validated method can be successfully applied to quantify the eleven investigated components in 22 samples of S. chinensis from different sources. |
