| ORIGINAL ARTICLE |
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| Year : 2012 | Volume
: 8
| Issue : 29 | Page : 4-11 |
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Application of deoxyribonucleic acid barcoding in Lauraceae plants
Zhen Liu1, Shi-Lin Chen2, Jing-Yuan Song2, Shou-Jun Zhang3, Ke-Li Chen4
1 Department of Pharmacy, The 309th Hospital of Chinese People's Liberation Army, Beijing; Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription, Ministry of Education, Hubei University of Chinese Medicine, Wuhan, Republic of China
2 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing, Republic of China
3 Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Republic of China
4 Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription, Ministry of Education, Hubei University of Chinese Medicine, Wuhan, Republic of China
Correspondence Address:
Ke-Li Chen
Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription, Ministry of Education, Hubei University of Chinese Medicine, Wuhan 430065
Republic of China
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0973-1296.93301
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Background: This study aims to determine the candidate markers that can be used as DNA barcode in the Lauraceae family. Material and Methods: Polymerase chain reaction amplification, sequencing efficiency, differential intra- and interspecific divergences, DNA barcoding gap, and identification efficiency were used to evaluate the four different DNA sequences of psbA-trnH, matK, rbcL, and ITS2. We tested the discrimination ability of psbA-trnH in 68 plant samples belonging to 42 species from 11 distinct genera and found that the rate of successful identification with the psbA-trnH was 82.4% at the species level. However, the correct identification of matK and rbcL were only 30.9% and 25.0%, respectively, using BLAST1. The PCR amplification efficiency of the ITS2 region was poor; thus, ITS2 was not included in subsequent experiments. To verify the capacity of the identification of psbA-trnH in more samples, 175 samples belonging to 117 species from the experimental data and from the GenBank database of the Lauraceae family were tested. Results: Using the BLAST1 method, the identification efficiency were 84.0% and 92.3% at the species and genus level, respectively. Conclusion: Therefore, psbA-trnH is confirmed as a useful marker for differentiating closely related species within Lauraceae. |
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