Pharmacognosy Magazine

ORIGINAL ARTICLE
Year
: 2017  |  Volume : 13  |  Issue : 49  |  Page : 7--12

Wheatgrass extract ameliorates hypoxia-induced mucin gene expression in A549 cells


Ju hwan Sim1, Moon-Hee Choi2, Hyun-Jae Shin2, Ji-Eun Lee1 
1 Department of Otorhinolaryngology-Head and Neck Surgery, Chosun University College of Medicine, Gwangju, Korea
2 Department of Biochemical and Polymer Engineering, Chosun University, Gwangju, Korea

Correspondence Address:
Ji-Eun Lee
Department of Otorhinolaryngology- Head & Neck Surgery College of Medicine, Chosun University, 365 Plimundaero, Dong-gu, Gwangju
Korea

Background: Wheatgrass is known to have antioxidant, antiaging, and anti-inflammatory effect. However, its protective effect against hypoxia is not yet evaluated. Objective: In this study, we evaluated the protective and anti-inflammatory effect of wheatgrass against the hypoxia in airway epithelial cells. Materials and Methods: A549 human lung adenocarcinoma cells were incubated in a hypoxic condition (CO2 5%/O2 1%) for 24 hr in the presence of different concentration of wheatgrass 50, 75, 100, and 150 μg/mL, and the magnitude of each immunologic response produced by the A549 cells was compared. The mRNA expression level of mucin gene (MUC), 5A, 5B, 8, GM-CSF, TNF-α, and VEGF were evaluated by using real-time polymerase chain reaction. The MUC proteins level before and after knocking out the hypoxia-inducible factor (hif)-1α via short interfering (si) RNA transfection were assessed by immunoblot analysis. Accordingly, the involved cell signaling pathway was evaluated by immunoblot analysis. Results: The inflammatory cytokines (GM-CSF, TNF- α) and the expressions of MUC 5A, 5B, and 8 were augmented by hypoxia. The augmented MUC expression was decreased by the wheatgrass extract administration. Hif-1α gene expression after hypoxia exposure was decreased by wheatgrass. Knockdown of hif-1α by siRNA reduced the mucin gene expression and which was more enhanced by wheatgrass extract. Conclusion: Theses results suggest that wheatgrass may be useful in the treatment of sinonasal disease by inhibiting mucus hypersecretion in airway epithelium. Abbreviations used: A549: human lung adenocarcinoma cells, GM-CSF: granulocyte-macrophage colony stimulating factor, HIF: hypoxia inducible factor, IL: interleukin, MUC: mucin, MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, TNF: tumor necrosis factors, VEGF: vascular endothelial growth factor, si RNA: short interfering RNA


How to cite this article:
Sim Jh, Choi MH, Shin HJ, Lee JE. Wheatgrass extract ameliorates hypoxia-induced mucin gene expression in A549 cells.Phcog Mag 2017;13:7-12


How to cite this URL:
Sim Jh, Choi MH, Shin HJ, Lee JE. Wheatgrass extract ameliorates hypoxia-induced mucin gene expression in A549 cells. Phcog Mag [serial online] 2017 [cited 2017 Feb 22 ];13:7-12
Available from: http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=49;spage=7;epage=12;aulast=Sim;type=0