Home | About PM | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2010  |  Volume : 6  |  Issue : 22  |  Page : 116-119

Determination of oleanolic acid and ursolic acid contents in Ziziphora clinopodioides Lam. by HPLC method


Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi-830011, Xinjiang, College of TCM, Xinjiang Medical University, Urumqi-830011, Xinjiang, China

Correspondence Address:
Shuge Tian
Experimental Teaching Demonstration Center of TCM in Xinjiang Medical University, Department of Traditional Medicine, TCM, Xinjiang Medical University, Xinjiang 830054
China
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-1296.62898

Rights and Permissions

A simple, precise, rapid and accurate, binary-phase high performance liquid chromatographic method has been developed for the determination of oleanolic acid and ursolic acid contents in the Ziziphora clinopodioides Lam. with short run time. Chromatographic separation is achieved by using HPLC system consisting of a Shimadzu LC-6AD and Kromasil C 18 column (150 Χ 4.6 mm, 10 ΅m, with pre-column), the mobile phase consists of methanol and 0.03 M phosphate buffer (pH = 3, 90:10). Detection wavelength is 214 nm. The speed of flow is 0.5 ml/min. The specimen handing quantity is 10 μl. The oleanolic acid's linearity range is 0.4~1.2 mg/ml (r = 0.9996). The ursolic acid's linearity range is 0.6~1.8 mg/ml (r = 0.9996), and the linear relationship is accurate. The average recovery (n = 6) of oleanolic acid is 99.5% (RSD = 1.19%) and ursolic acid is 102.3%(RSD = 1.25%). The content of oleanolic acid and ursolic acid in Ziziphora clinopodioides are 0.76 mg/g and 1.176 mg/g, respectively. The developed HPLC method can therefore be applied to both in vitro studies of oleanolic acid and ursolic acid formulations as well as drug estimation in biological samples.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed5946    
    Printed216    
    Emailed5    
    PDF Downloaded181    
    Comments [Add]    
    Cited by others 21    

Recommend this journal