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ORIGINAL ARTICLE
Year : 2010  |  Volume : 6  |  Issue : 22  |  Page : 106-110

Rapid determination of polysaccharides in BianTi Soft extract by spectrophotometry coupled with gas chromatography-mass spectrometry


1 Traditional Chinese Medical Hospital of Zhejiang Province, No. 54 Youdian Road, Hangzhou, Zhejiang Province - 310 006, P.R, China
2 The First Affiliated Hospital of Medical School of Zhejiang University, No. 79 Qingchun Road, Hangzhou, Zhejiang Province - 310 003, P.R, China

Correspondence Address:
Jie Shen
Traditional Chinese Medical Hospital of Zhejiang Province, No. 54 Youdian Road, Hangzhou, Zhejiang Province - 310 006, P.R
China
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DOI: 10.4103/0973-1296.62895

PMID: 20668575

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A simple approach for the rapid determination of polysaccharides in BianTi Soft Extract using spectrophotometry coupled with gas chromatography-mass spectrometry (GC-MS) was developed. The mixed standard solution composed of D-glucose, D-mannose, galactose and D-xylose in different proportions (1.00: 1.01: 0.12: 0.05) was prepared according to the monosaccharide composition analysis of the polysaccharides by GC-MS. The determination of polysaccharides by UV-Vis spectrophotometer was performed after 35-min color reaction, in which 1 ml 5% phenol and 4 ml sulfate was used. The assay of the method validation has shown that the method was stable, reliable and feasible. Furthermore, the proposed method was successfully applied in the preparation procedure of BianTi Soft Extract, selecting out optimal decoction conditions and suitable decoction container. It suggests that the convenient method could be useful for the quality control of BianTi Soft Extract. Meanwhile, it may be an alternative for polysaccharides determination of other formulations.


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